US has confirmed the ID of Osama by DNA test.
Taking into account the manner of his death in an alien country and the reported burial of the body into the sea,the time factor involved in identifying seems to suggest that he might have been killed earlier.
How and when the body was transported and to where?
Procedures for DNA Test.
Extract DNA
If they’ve got his body, then they’ve got enough DNA to run a billion or two genetic tests. It takes extremely little DNA to run genetic tests – on the order of single cells. So having even a 1 mm square piece of flesh would provide more DNA than they would even have use for. Extraction takes very little time. All you need to do it place the cells/tissue in some kind of solution that will break up the cell’s membranes, thus liberating the DNA from the nucleus without damaging the DNA too much. There are hundreds of extraction kits and protocols. I don’t know what the gov’t extraction policy is, but the Arkansas State Crime Lab just uses sodium hydroxide and hydrochloric acid to extract DNA from their samples (which, btw, is how I get DNA from my fish samples, too).
1. Place 52 μl of 0.01 M NAOH in each well with 2.0 mm of tissue.
2. Incubate samples at 65°C for 10 minutes.
3. Add 10 μl of 0.1 M Tris HCl (pH 7.3).
4. Mix.
5. Let stand for 5 minutes.
6. Samples are now ready for amplification
TOTAL TIME: 15 min
Once you have DNA, you’re ready to ID your suspect. While there have been a few methods used in the past, the onset of Polymerase Chain Reaction, or PCR technology, has made looking at parts of a genome pretty darn quick and easy.
The namesake of PCR, polymerase, is a very special enzyme that cells use to duplicate DNA. Polymerases are found in all creatures because we all must, at some point, have cells divide to grow and reproduce. When our cells divide, we have to make two copies of our genome—one for each new cell. To do this, our cells unwind the DNA, spread apart the two matching strands, then use each as templates to make two new strands. Polymerases are the enzymes that actually do that—they attach to single strands of DNA and grab matching nucleotides to create the other half of the strand.
Ok perfect, with what kind of primers you do PCR